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rabbit anti asc  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit anti asc
    Rabbit Anti Asc, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+asc/pm41931855-78-37-40
    Average 86 stars, based on 1 article reviews
    rabbit anti asc - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Western Blot:

    Article Title: Hesperetin alleviates neuronal pyroptosis by promoting mitophagy via DHCR24/BACE1 signaling pathway after subarachnoid hemorrhage in mice.
    Article Snippet: Caspase-1 Cysteinyl aspartate-specific proteinase-1 DHCR24 3-β-Hydroxysteroid-Δ-24-reductase DCFH-DA 2′,7′-Dichlorofluorescin diacetate DAPI 4’,6-Diamidino-2-phenylindole EBI Early brain injury ECL Electro-chemi-luminescence EPM The Elevated Plus Maze GSDMD Gasdermin D IL-18 Interleukin-18 NOR The New Object Recognition test OFT Open field test OxyHb Oxyhemoglobin PBS Phosphate buffer saline PVDF Polyvinylidene Fluoride PFA Paraformaldehyde ROS Reactive oxygen species SAH Subarachnoid hemorrhage TME Transmission electron microscope WB Western blot Abbreviations ASC Apoptosis associated with speck-like proteins BACE1 Beta-site amyloid precursor protein cleaving enzyme BBB Blood–brain barrier

    Article Title: Inflammasome adaptor ASC promotes sustained neuroinflammation and mild cognitive impairment in a closed-head injury model
    Article Snippet: .. For immunoblotting, the following primary antibodies were used: mouse anti-CASP1 (1:1,000; AdipoGen; AG-20B-0042); rabbit anti-ASC (1:1,500; Cell Signaling Technology; D2W8U); rabbit anti-NLRP3 (1:500; Cell Signaling Technology; D4D8T); rabbit anti–IL-1β from (1:1,000; E7V2A; Cell Signaling Technology); rabbit anti-CASP3 (1:1,000; D3E9; Cell Signaling Technology); and rabbit anti-CASP8 (1:1,000; D5B2; Cell Signaling Technology). .. For immunohistochemical analysis, the antibodies included rabbit anti-Iba1 (1:1,000; Fujifilm Wako reagents, 019-19741); goat anti-Iba1 (1:1,000; Abcam; EPR16588 ); rat anti-GFAP (1:300; Thermo Fisher Scientific; 2.2B10); goat anti–IL-1β (1:500; R&D Systems; AF-401-SP), rabbit anti-ASC (1:1,500; Cell Signaling Technology; D2W8U); and rabbit anti-CASP3 (1:1,000; D3E9; Cell Signaling Technology).

    Incubation:

    Article Title: IL-38 reprograms macrophage metabolism to restrain NLRP3 inflammasome assembly and attenuate monosodium urate crystals induced inflammation.
    Article Snippet: Objective: Although interleukin (IL)-38 is recognized as an anti-inflammatory cytokine, its precise mechanism in monosodium urate (MSU) crystals induced inflammation remains undefined, and its impact on macrophages metabolism during MSU crystals induced inflammation has not been explored.. Method: RAW264.7 cells, THP-1 cells and mouse peritoneal macrophages were stimulated with MSU crystals with or without recombinant IL-38.. Glucose uptake, Glucose Transporter Type 1 (GLUT1) expression, Reactive Oxygen Species (ROS) levels and NOD-like receptor family, pyrin domain-containing protein 3 (NLRP3) inflammasome activation and assembly were assessed by quantitative real-time PCR, immunofluorescence and western blot.

    Article Title: Aucubin attenuates neonatal hypoxic-ischemic brain injury by suppressing NLRP3 inflammasome-mediated microglial pyroptosis.
    Article Snippet: Clinical treatment of neonatal hypoxic–ischemic encephalopathy (HIE) remains a major challenge for neonatologists, and alternative or complementary treatments are urgently needed.. The pathogenesis of HIE is complex, and active ingredients derived from natural medicines, characterized by their multitarget effects and coverage of multiple mechanisms, are attracting increasing research attention.. Aucubin has been reported to have anti-inflammatory, antioxidant and antitumor effects, but its specific role and underlying mechanism in HIE treatment require elucidation.

    Control:

    Article Title: IL-38 reprograms macrophage metabolism to restrain NLRP3 inflammasome assembly and attenuate monosodium urate crystals induced inflammation.
    Article Snippet: Objective: Although interleukin (IL)-38 is recognized as an anti-inflammatory cytokine, its precise mechanism in monosodium urate (MSU) crystals induced inflammation remains undefined, and its impact on macrophages metabolism during MSU crystals induced inflammation has not been explored.. Method: RAW264.7 cells, THP-1 cells and mouse peritoneal macrophages were stimulated with MSU crystals with or without recombinant IL-38.. Glucose uptake, Glucose Transporter Type 1 (GLUT1) expression, Reactive Oxygen Species (ROS) levels and NOD-like receptor family, pyrin domain-containing protein 3 (NLRP3) inflammasome activation and assembly were assessed by quantitative real-time PCR, immunofluorescence and western blot.

    Membrane:

    Article Title: Aucubin attenuates neonatal hypoxic-ischemic brain injury by suppressing NLRP3 inflammasome-mediated microglial pyroptosis.
    Article Snippet: Clinical treatment of neonatal hypoxic–ischemic encephalopathy (HIE) remains a major challenge for neonatologists, and alternative or complementary treatments are urgently needed.. The pathogenesis of HIE is complex, and active ingredients derived from natural medicines, characterized by their multitarget effects and coverage of multiple mechanisms, are attracting increasing research attention.. Aucubin has been reported to have anti-inflammatory, antioxidant and antitumor effects, but its specific role and underlying mechanism in HIE treatment require elucidation.



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